發佈日期：2012/04/19 am10:29:15

內容：
Anthocyanins are known to have antioxidant effects and thus may play an important
role in preventing various degenerative diseases. In this study, we examined the
effect of anthocyanins extracted from the seed coat of black soybean on an animal
model of retinal degeneration (RD), a leading cause of photoreceptor cell death
resulting in blindness. RD was induced in rats by an intraperitoneal injection of
N-methyl-N-nitrosourea (MNU) (50mg/kg), a DNA-methylating agent that causes
photoreceptor damage. Anthocyanins extracted from black soybean seed coat
(50mg/kg) were daily administered, orally, for 1, 2, and 4 weeks after MNU
injection. Electroretinographic (ERG) recordings and morphological analyses were
performed. In control rats with MNU-induced retinal damage, the ERG recordings
showed a gradual significant time-dependent reduction in both a- and b-wave
amplitudes compared with those of normal animals. In the MNU-induced RD rats
given anthocyanins for 4 weeks, ERG responses were significantly increased
compared with untreated RD rats, more apparently in scotopic stimulation than in
the photopic condition. However, in the MNU-injected rats given anthocyanins for
1 and 2 weeks, the increase in ERG responses was not significant.
Morphologically, the outer nuclear layer, where photoreceptors reside, was well
preserved in the anthocyanin-treated rat retinas throughout the experimental
period. In addition, retinal injury, evaluated by immunolabeling with an antibody
against glial fibrillary acidic protein, was markedly reduced in
anthocyanin-treated retinas. These results demonstrate that anthocyanins
extracted from black soybean seeds can protect retinal neurons from MNU-induced
structural and functional damages, suggesting that anthocyanins from black
soybean seed coat may be used as a useful supplement to modulate RD.


Copyright © 2012. Published by Elsevier Ltd.

PMID: 22387136 [PubMed - as supplied by publisher]

中文簡述：
花青素具有抗氧化作用，從而可能在預防各種眼睛退化性疾病上發揮了重要作用，在這項研究中，我們研究了在動物模型視網膜病變的大鼠身上，因感光細胞死亡造成失明，進而了解對從黑大豆種皮中提取的花青素的效果。從黑大豆種皮（50mg/kg的）中提取的花青素的日常管理，藉由口服的方式，1、2、4週後的MNU注射後，對電圖（ERG）的反應和形態分析進行。在對照組的MNU誘導的視網膜損傷，視網膜電圖記錄表明一個漸進的兩個A級和B波振幅與正常動物相比有顯著的時間依賴性減少。在對甲基亞硝基脲誘導RD老鼠4週的花青素，ERG的反應均顯著增加，與未經處理的RD大鼠，在暗的刺激更為明顯比起白天明視條件。這些結果表明，黑大豆種子中提取的花青素可以保護視網膜神經細胞的MNU誘導的結構和功能的損害，這表明黑大豆種皮花青素可以作為人體有益的補充保健品。